As you follow the on-screen instructions, you can create new batches to suit your hardware configuration and sample type. The following screens are displayed. Note that different screens may be displayed depending on your hardware configurations and the items selected.
[Welcome to ICP-MS MassHunter Method Wizard] dialog box
Check that the correct system configuration is displayed, and then click [Next].
If your system configuration differs, click [Cancel], and then set your configuration in the [Settings] dialog box.
This dialog box is only displayed when "Single Nanoparticle Application Module" is activated.
Select appropriate Analysis Mode.
Conventional Analysis
[Spectrum/TRA]
Spectrum：Conventional Mass Spectra are acquired.
TRA：Conventional Time Resolved Data are acquired.
Nanoparticle
[Single Element / Isotope]
Single Particle Analysis using fast TRA mode. However, the minimum sampling period is 3 ms because the 7700 and 8800 do not support Fast TRA.
[Rapid Multi-Element Nanoparticle Analysis]
Fast Time Program Analysis for multi-element nanoparticle screening. You can acquire a maximum of 16 Elements/Isotopes.
The Agilent 7700 and 8800 do not support this function.
You can set 17 or more tune modes. To use this feature, turn on [sNP Extended Functions] from the [Add-Ins] group on the [Tools] tab, and turn on [Extended Acquisition] from the [sNP Extended Functions] group on the [sNP Extension] tab displayed.
[2 Elements / Isotopes]
Average element/isotope ratios of multiple particles.
[FFF ICP-MS]
Particle are acquired using Field-Flow Fractionation.
This item is displayed when [Advanced Acquisition] is selected in the [Options] category in the [Settings] dialog box.
You need to select [Advanced Acquisition] (Option).
[Method Setup Assistant] dialog box
This dialog box is displayed when meeting all of the following conditions:
[IntelliQuant Assistant]
Use IntelliQuant Assistant to automatically optimize the best conditions for your selected elements.
[Preset Method]
Conventional Mass Spectra / Time Resolved Data are acquired.
[Method Wizard Mode] dialog box
This is displayed when using the x-lens for the 7900 and the Status is Online.
Select [Manual] mode or [Automatic] mode.
[Manual]: Create optimized method using typical parameters based on user input. Data acquisition will not be done.
[Automatic]: Create optimized method automatically based on acquisition of a blank, reference standard and typical sample. Instrument need to be tuned with startup tasks.
When the method automation process ends successfully, a method automation report is displayed. The text file is stored as “Method\MethodAutomation.csv” in the selected method automation batch folder.
[Plasma Robustness] dialog box
Select Matrix. Plasma condition will be set accordingly, and appropriate preset methods will be presented. Click [Next].
[Preset Method] dialog box
Select your current method from the [Preset Method] list. Those preset methods are available which meet your hardware configuration and matrix selection.
If no preset method is selected, you will be prompted to enter analyte and internal standard information.
[ISTD Stock Solution] dialog box
These items are displayed when a preset method is not selected. Select ISTD stock solution and ISTD element.
[Element List] dialog box
These items are displayed when a preset method is not selected. You can import the element list from the preset method. If you do not want to use the current method, you can configure a different method the [Elements] dialog box.
[REE++ Correction] dialog box
Users can select the mode used to correct interference from “REE++ Correction” or “Acq.Defined” on the REE++ Correction page. This page is displayed in Method Wizard under the following conditions:
[REE++ Correction]:
Use the interference correction automatically calculated by REE++ isotopes from the half-mass acquisition data for As, Se, or Ba.
If “REE++ Correction” is selected, the half-masses necessary for correction are added to the acq parameters automatically. However, they are not added to the Analyte List in the data analysis method. This means that they are not analyzed and are only used for correction.
[Acq. Defined]:
Use the conventional interference correction method from the acquisition data.
You can only run corrections for batches created with “REE++ Correction” in the Method Wizard.
You cannot select TRA when the batch is created with “REE++ Correction”.
For detailed information on the calculation formula, refer to REE++ Correction.
[Elements] dialog box
These items are displayed when a preset method is not selected. Select or edit elements.
If you select any elements present in your ISTD as analyte elements, method automation will automatically calculate them as ISTD elements.
[Single Particle Analysis Configuration] dialog box
Displayed when you select [Single Element / Isotope] in the [Analysis Mode] dialog box.
Sample Pump Tube ID
Select the Sample Pump Tube ID you want to use.
Sample Inlet Flow
Measures the sample flow from Sample Pump Tube ID and enters it automatically. You can also calculate this in the [Agilent ICP-MS Flow and Dilution Factor Calculator] dialog box and enter it manually.
Response Factor Calibration Solution
Ionic Standard Concentration at XXX amu
Input a concentration of Ionic Standard. The default value is 1 ppb.
Reference Material
Select Reference Material from the list. The parameter for the selected Reference Material is entered automatically. When using a Reference Material other than those shown in the list, select [Custom] and enter the following parameters.
Reference Element Mass
Represents the mass of the standard element within the reference materials.
Mean Reference Particle Diameter
Average diameter of standard particles is suspension.
Reference Element Density
Material density of reference material. This depends of the elemental composition. For example, Au = 19.30 g/ml, Ag = 10.49 g/ml, SiO2 =2.634 g/ml, TiO2 = 4.23 g/ml, ZnO = 5.606 g/ml.
Mass Concentration of Reference Material
Mass concentration of standard particles in suspension.
Unknown Sample
Target Element Mass
Displays the mass number for the target elements in Unknown Sample.
Analyte Mass Fraction
This is the ratio between molar mass of the particle material and molar mass of the analyte being measured.
Particle Density
Enter the particle density of unknown sample.
[Analyte Element] dialog box
Displayed when either mode is selected in the [Nanoparticle] in the [Analysis Mode] dialog box.
The element to be analyzed is set.
Select a single element for the [Single Element / Isotope], and then select two elements for the [2 Elements / Isotopes].
[Import Reference Sample and Calibration Samples] dialog box
Displayed when [Single Element / Isotope] is selected in the [Analysis Mode] dialog box.
To import a Reference Sample from the existing batch, select either [Existing Batch] or [Previous Batch] from the [Import Reference Sample and Calibration Samples] pull-down menu.
[Analytes] dialog box
These items are displayed when the preset method is selected. Select or edit Analyte and Internal Standard Isotopes.
[Integration Time] dialog box
These items are displayed when the preset method is selected. Edit Integration Time/Mass.
[Optimize Performance] dialog box
Select whether you optimize the performance of this method and the criteria either "Speed" or "Low DL". Click [Optimize].
[Method Wizard has finished creating the method] dialog box
Click [Finish] to proceed with creating a batch based on your new method.
You will be able to use your newly created batch directly, or save it as a template for future use.